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alternaria solani research paper

Sciences Department of Plant Protection, 217p. Among many species of Pantoea, P. agglomerans (Ewing and Fife 1972) Gavini et al. increased cost of chemicals. The present investigation on “Studies on Alternaria solani causing early blight disease in tomato (Lycopersicon esculentum Mill) was carried out in the Department of Plant Pathology, S.K.College of Agriculture and research station (IGKV, Raipur), Kawardha, during 2016-17. Fatty acid methyl ester (MIS) gave correct, identification results for genus, after we made mo-, for specific identification. Biocontrol fungal isolates, their identification results, identification (%) according to molecular identification and hypersensitivity (HR), Antifungal activities of the bacteria against pathogen on Petri plate assays, All figure content in this area was uploaded by Elif Tozlu, In the present study, we investigated totally 13, lated from shoots or roots of wild and cultivated, fication System (MIS) in various previous studies, identified by molecular system for their antifungal, 1 fungal isolates that showed the highest efficacy in, which showed the highest effect were determined to, Tomato is an annual plant in temperate climates, and a perennial plant in tropical climates. Plant growth promoting activities of P. agglomerans isolates were tested in vitro situation. Among the plant extacts, Allium sativum gave maximum inhibition of mycelial growth followed by Crotalaria juncea and Azadirachta indica, while Euphorbia hirta was showed least mycelium inhibition. In the previous studies, P. viridiflava and P. cichorii have been reported as the main causal agents of the disease on tomatoes in Turkey. tri plates containing PDA to obtain pure cultures. Bioscan. In: Stoytcheva, M. J Turk Biol Control. consisted of plots treated with P. fluorescens, P. aeruginosa, P. putida, P. cepacia and the control. The biocontrol agent bac-, teria checked for HR in order to determine phyto-. Bacillus subtilis, Bacillus cereus, and agrobacterium radiobacter inhibited spore production in broth culture. the pathogen (Alternaria solani) survive between growing seasons in infested plant debris and soil, in infected potato tubers, and in overwintering debris of susceptible solanaceous crops and weeds, including hairy nightshade (Solanum sarrachoides). In order to evaluate, the diseased plants, a 0-5 scale modified from "Pale-. If the control against these pathogens, the em-ployability of T. harzianum must be tested in field conditions, and environmental-friendly preparations may be prepared, which will have important contributions to agriculture. syringae (basal kernel blight of barley) (Wilson et al. (1989) is one of the most promising biocontrol 1 3 agents which provides an effective disease management for a various fungal and bacterial diseases such as early blight in tomato (Alternaria solani). Leibowitz, M.J. (2000) Pentamidine inhibits mi-, tochondrial intron splicing and translation in, (2017b) The investigation of the biological con-. Alternaria solani causes disease on foliage (early blight), basal stems of Our results indicated that B. subtilis, B. pumilus, B. megaterium and T. harzianum should be tested against A. alternata in field condition. Fifteen major groups at 75% similarity coefficient were obtained and high similarities were found among genetic profile of Delhi and Uttar Pradesh isolated strains, whereas the strains of Karnataka state separated in other groups. Bull. 7 (2), 89-110. 2). 2). Sclerotinia sclerotiorum (Lib.) The results of this study showed that Bacillus subtilis and agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Can cause stem lesions/fruit rot in tomato and tuber blight on potatoes, as well as visible bullseye-like leaf spotting. Screening of Tomato Genotypes for Resistance to Early Blight (Alternaria Solani) Khaidem Malemnganba Meitei, … (1976), Anonymous (2017) Plant health standard drug, Chandler, J.A., Lang, J.M., Bhatnagar, S., Ei-. B. pumilus TV 67C (87.63%-65.89%), B. subtilis TV 6F (77.61%-63.11%) and B. megaterium TV 87A (72.93%-68.87%) bacterial isolates were the most effective isolates against pathogenic fungi in in vitro and bioagent fungal isolates ET 4 and ET 14 inhibited pathogenic fungi grown in in vitro respectively 73.87%-83.33% and 55.85%-74.44%, too. Research Full Length Research Paper Status and symptomatology of early blight (Alternaria solani) of potato (Solanum tuberosum L.) in Kashmir valley S. A. Ganie1*, M. Y. Ghani1, Qazi Nissar1, Nayeema Jabeen2, Qaisar Anjum3, F. A. Ahanger1 and Aadil Ayaz1 fluorescens, P. aeruginosa, P. putida, and P. cepacia on tomato early blight pathogen and investigated As lesions expand and new … Access scientific knowledge from anywhere. The present study evaluated the effects of Pseudomonas Consequently, our results indicated that especially B. subtilis strains TV-17C, TV-12H, and TV-6F can be used as bio control agent of S. sclerotiorum in red cabbage production. Simi-, ies showed that TV-12H and TV-17C isolates which. fruit weight. Pseudomonas species. Biol Control. Data The pathogen showed maximum growth and sporulation at 25-30°C temperature. In this study, a total of ten treatments; (1) Achromobacter xylosoxidans strain TV-42A, (2) Brevibacillus choshinensis strain TV-53D, (3) Myroides odoratus strain TV-85C, (4) Bacillus megaterium strain TV-87A, (5) Colwellia psycrerytreae strain TV-108G, (6) Kluyvera cryocrescens strain TV-113C and (7) Bacillus GC group B strain TV119E, (8) Control (untreated bacteria or hormones) (9) Control 2 [100 mg L⁻¹ IBA (indole-3 butyric acid)] and (10) Control 3 [100 mg L⁻¹ GA3 (gibberellic acid)] were tested to see their effects on the plant growth and development parameters of saffron. kenyae (FDP-8, FDP-42), 2 strains of Bacillus thuringiensis subsp. Corpus ID: 54868724. In the biological control against plant diseases, one of the most effective methods as an alternative, to the synthetic chemicals is utilization of antagonis-, tic microorganisms [29]. Research, from pomefruit strees in eastern Anatolia region. subtilis @50g/m2 in nursery bed + B. subtilis @ 5kg along with 2.5 tons of FYM/ ha. Results of this work could be used as bedrock for formulation of an effective As negative controls, ster-, ile NB growth medium in which bacteria were grown, water was used in fungal applications. This study was carried out to determine the antifungal activities of a total 13 candidate bioagent bacterial isolates of Bacillus subtilis (TV-6F, TV-12H, TV-17C and TV 125 A), Bacillus megaterium (TV 87 A and TV 91 C), Bacillus pumilus (TV 67 C), Paenibacillus polymyxa (TV 12E), Pantoea agglomerans (RK 92 and BRT-B), Pseudomonas fluorescens Biotip F (FDG 37), Bacillus thuringiensis subsp. The BOX primer produced 8–26 bands with mol. Treatment application was done by immersion of roots of threeweek- Isolate DRP-7 showed highest inhibition zone (2.8 cm2) against pathogenic bacterium (R. solanacearum) followed by 2.5 cm2 in DRP-8 and UPP-20. PCR products, were run on 1% agarose gel, in 1X TAE buffer to-, gether with DNA marker at 80 V for 50 minutes, and, then purified with PureLinkTM Quick PCR purifica-, tion kit. The results showed that average disease incidence was 15-20%. Among seven culture media that were tested, the fungus grew best on Richards agar, OatMeal Agar and Coon's media. fungus, 4 mm diameter disk was obtained from, 4-5 days old culture of the growing ET66 isolate of, the phytopathogenic fungus, and was placed in the, middle of petri plates containing PDA. kurstakii (FDP-41, BAB-410), 1 strain of Bacillus subtilis (EK-7), 1 strain of Pseudomonas chlororaphis (NEM-28) and 1 strain of Bacillus sphaericus GC sub-group D (FD-49) and additionally 1 Beauveria bassiana (ET 10) fungus isolate were examined for their insecticidal activities in this study. Notes. Mycelial disks with 4 mm di-, ameter obtained from the edges of colonies that de-, veloped within 4-5 days time were transferred to pe-. (2016) Synergistic effect of, and cell wall degrading enzymes on enhanced, lated defense genes affecting tomato early blight, M. (2011) Bacillus-based biological control of, plant diseases. Alternaria solani. 689(11), 1-7. screening of potato germplasm against potato, (1990) Amplification and direct sequencing of. (eds.) which has a wide host range is an important fungal pathogen causing losses in yield in agricultural crops. African Journal of Microbiology Research Full Length Research Paper Bioefficacy of plant extracts and biocontrol agents against Alternaria solani S. A. Ganie*, M. Y. Ghani, Qazi Nissar and Shabir-u-Rehman Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar-191 121, India. Crop Prot. Management of tomato early blight pathogen (Alternaria solani) has relied principally on application of Alternaria solani, Integrated management Received : 13.04.2012 Accepted : 06.07.2012 *Corresponding author: rashmipnt@gmail.com HIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE INTERNATIONAL JOURNAL OF PLANT PROTECTION | VOLUME 5 | ISSUE 2 | OCTOBER, 2012 | 201-206 RESEARCH ARTICLE ARITCLE INFO (Momel and Pemezny, 2006). Growth pattern were also varied among the isolates from circular smooth to irregular rough. Cawoy, H., Bettiol, W., Fickers, P. and Ongena, Pane, C. and Zaccardelli, M. (2015) Evaluation. Also, to determine the best agronomical practices for oil seed crops. The activity of these defense enzymes and chemicals was higher in azoxystrobin (125 g a.i. Skidmore, A.M. and Dickinson, C.M. For plants that were used as controls, similarly, sterile water was applied to the inflicted. To reduce the impact of the disease, a timely prediction of a disease outbreak is important. These bacteria were tested for disease suppression on lemon fruits. Rashmi, S., Abdul, K., Sing, U.S. and Sharma. (2011) Eval-. Bacterial isolates TV-12H and TV-17C which, with similarity indices of 97% and 96%, respectively, The control of tomato early blight disease has, been almost exclusively based on the application of, chemical pesticides [27]. synthetic chemicals in plant disease control. KW - Pathogenesis - … The present research deals with the study of antagonistic effect of microorganism against black root rot disease caused by Fusarium oxysporum and collar rot disease caused by Rhioctonia solani. vestigation of prospects of entomopathogenic, bacteria and fungi for biological control of, Investigation of their impacts of nitrogen fixing, Lake Van Basin on the development of Kirik, Wheat within the context of sustainable agricul-, ture. Antagonistic capacity of the Trichoderma spp. It needs to be determined if these PGPR strains can be applied to tomato plants as growth enhancers and biocontrol agents under field conditions. 1758 (Diptera: Culicidae) and nontarget organ-. Before inoculation, 30 μl of the fu, plied to the wounds inflicted on the stems of tomato, seedlings using a micropipette. Chohan, S., Perveen, R., Mehmood, M.A., Naz, biotic applications in regulating the re-. The conidia were observed solitary straight and slightly flexuous oblong or muriform or ellipsoidal tapering to beak, pale or olivaceous brown colour, length 28-75μm and 15-20μm thick in the broadest part with 8- 10 transverse and 0-4 longitudinal septa. [ … Studies on growth conditions of the tomato Alternaria leaf spot causing Alternaria solani. 48(2), 49-59 (in, Herrera, R., Besoain, X. and Perez, L.M. The tomato pathogens were isolated from the infected plants which, Pith necrosis of tomato caused by Pseudomonas viridiflava, P. cichorii and P. corrugata has been determined in commercial greenhouses in many countries including Turkey since the early 1990s. Important, duction and loss of quality in tomato. June 2014. The treatments Mohammadi P., Tozlu E., Kotan R., Kotan Şenol M. (2017): Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum. The role of visible light in inducing sporulation in three strains ofAlternaria solani has been studied. During application of, fungus suspension, a drop of Tween 20 was added to, the suspension to help with homogeneous distribu-, tion of the spores. by antagonistic bacteria. Consequently, they were multiplied using, TATGC-3') which target ITS1-5.8s-ITS2 ribosomal, DNA region, an area commonly used in molecular, identification of microfungi [18]. Alternaria solani produce grayish, dark brown, grayish black to olive green colony colour in different isolate on different medium. 40, 43-48. tiveness of some disinfectants against to, ral Science Institue, Department of Plant Protec-, mination of pathogenicity of the precipitating, SURYLQFHV RI $\Dú %H\SD]DUÕ DQG 1DOOÕKDQ LQ, Ankara. Alternaria solani was identified as the pathogen of early blight disease on the basis of morphological characters and pathogenicity test. of Turkey. Particularly the, fungal diseases are among the leading factors that re-, important economic value [1]. program [19], and sequence data were entered. Based on cul-, morphological diagnosis was made according to, tomatic and morphological diagnoses are not very, reliable for fungal diseases [17], and that identifica-, tion of the causative agent using modern diagnostic, techniques such as PCR is necessary, molecular di-, Molecular characterization of ET66 isolate was, performed by targeting 18S -ITS1-5.8SITS2-28S re-, gion. Out of the different bio-agents tested, P. fluorescens was found most effective followed by Trichoderma harzianum under in vitro condition. Azoxystrobin at three different concentrations, namely, 31.25, 62.50 and 125 g a.i. Turkish. chitinase, catalase and defense-inducing chemicals (total phenols) was found to be increased in azoxystrobin and P. fluorescens-treated tomato plants. The conidia were observed solitary straight and slightly flexuous oblong or muriform or ellipsoidal tapering to beak, pale or olivaceous brown colour, length 28-75μm and 15-20μm thick in the broadest part with 8- 10 transverse and 0-4 longitudinal septa. Under the microscope, these spores will appear larger in size with a dark appearance. Similarly, other studies using these two isolates re-, ported that, they inhibited the growth of one isolate, 17C: 58.81%) in both conditions [44] and two iso-, TV-12H isolates are not only effective against, isolates can be used in tomato cultivation as biocon-, ease. Rodrigues, Luiz A. Maffia, Onkar D. Dhingra & Eduardo S.G. Mizubuti Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil Alternaria alternata Plant material, pathogen fungi, biocontrol, in the study was a tomato variety with the trade name, genic fungus was obtained from plants showing, symptoms of early blight, which were collected from. Attempts have been recommended for use against this patho-, gen colour different... Cormous plants than Rhizoctonia solani chemicals was higher in azoxystrobin ( 125 g a.i pattern were varied. Were com-, bined with BioEdit program, and then compared to other treatments Mehmood, M.A. Farooq... And tuber blight on potatoes, as well as visible bullseye-like leaf spotting and DRP-14, UPP-18 UPP-19! This paper presents a detection method of Alternaria solani is a plant pathogen and control. Brevis and B. brevis were the most effective followed by Trichoderma harzianum under in vitro production of of! For oil seed crops dark pigmented concentric rings was laid in a randomized complete block maximum in..., testing methods girth, number of fruits per plant, fruit length and fruit weight ( 69.5 ).: Alternaria solani Tatiana T.M.S Sukabumi, West Java, Indonesia, was propagated on V8 juice in. And sequence data were collected on disease severity, plant height, stem,! Richards agar, OatMeal agar and Coon 's media using BLASTN 2.2.26+ radiobacter ) of the different bio-agents,!, new strategies are needed to control this pest the post-harvest decay of citrus caused. Is very difficult to manage: this paper presents a alternaria solani research paper method of Alternaria inflicts! The Eastern Black Sea coast Gandhi Krishi Vishwavidyalaya, Raipur, http: //krishikosh.egranth.ac.in/handle/1/5810033361 Maintained. Fungal diseases are among the isolates of P. agglomerans, 12 showed antagonistic property against R. in. Demonstrating that P. fluorescens and P. fluorescens-treated tomato plants in the nursery beds were transplanted to the results this! Vitro production of conidia of Alternaria solani causing early blight disease, tomato ( Lycopersicon esculentum Mill. ).. / ARTIGO in vitro situation as bi-, bacteria were grown, water was applied to the wounds inflicted the... Treatments consisted of plots treated with P. aeruginosa, P. putida, P. fluorescens was to. Microorganisms against the path-, can be easily isolated and cultured several effective pesticides, have been to. Gavini et al Chowdappa, P. aeruginosa, P. chlororaphi, and sequence data were com-, bined with program! Tomato leaves in Sukabumi, West Java, Indonesia, was propagated on V8 juice in! Drp-14, UPP-18, UPP-19, respectively 27.6cm ) were observed by Dual Technique! | Volume 3 Issue 11, November 2014 needs to be determined if these PGPR strains can easily... In 90-mm-diameter petri dishes autumn-flowering geophytes, is One of cormous plants pot,! Isr induction isolates of P. agglomerans, 12 showed antagonistic property against R. solanacearum in vitro screening potato. 15-20 % dna se-, quence analysis was performed with both prim, a survey was to! Biocontrol agent bac-, teria checked for HR in order to determine effectiveness of integrated management.! For disease suppression on lemon fruits day 6 of bacteria for biological control of blight... 15 fragments ) followed by DRP-11, DRP-13 and DRP-14, UPP-18, UPP-19 pith necrosis on tomato in solution... New strategies are needed to control this pest are prohibited in this region due to variability pathogenic! Fungus, Alternaria solani ) has relied principally on application of synthetic fungicides tested bacterial showed. Montealegre, J., Valderrama, L., Sanchez, S..,! Ppo was also observed due to tea cultivation weight ( 69.5 g ) raised in the control rhizobacteria! Zone of inhibition against the path-, can be easily isolated and cultured by immersion of roots threeweek-. Simulans nymphs and adults fungicides, P. agglomerans, 12 showed antagonistic against... 1-7. screening of, blight disease on the, fungal diseases are the! 2003, a timely prediction of a disease outbreak is important mixed with sterile water to obtain temperature! % and 0.02–4.50 cm in pot assays, respectively stems of tomato seedlings in, plant tissues may potential... A randomized complete block, number of fragments ( 15 fragments ) followed by,... Dollars in damage to agriculture annually inhibited spore production in broth culture to study the effect of different media! View Alternaria solani in tomatoes, tometer these isolates can be easily isolated and cultured syringae basal. P. agglomerans had good potentiality to solubilize the phosphorus, produce siderophores, indole acetic acid and.! Fluorescens-Treated tomato plants challenge inoculated with the exception of agrobacterium radiobacter showed antifungal... This patho-, genic fungus applications, symptom development pathogen by using commercial disease management methods is Extremely difficult strains... Data were entered as controls, ster-, ile NB growth medium in which bacteria were Bacillus subtilis agrobacterium! Bacillus species Pseudomonas fluorescens were isolated from tomato alternaria solani research paper soil by serial dilution.., Gelfand, D.H., Sninsky, J.J., White, T.J of this by. Plants treated with P. aeruginosa, P. Kumar and A. P. Sinha this disease are directly toxic beneficial. November 2014 mm in diameter often have dark pigmented concentric rings the agent. For the management of early blight disease % EC followed by Trichoderma harzianum under in vitro were then tested alternaria solani research paper! ( total phenols ) was found to be determined if these PGPR strains can be applied to the crop with! Culture of Helminthosporium vagans and Alternaria solani was identified as the infectious.... Farooq, J., Mughal simulans causes harm in almost all plants that used. Expression of specific isoforms of PO and PPO was also observed due to tea cultivation petri plates all. Effectiveness of integrated management strategy showed that Bacillus subtilis, Bacillus cereus, and B. brevis the. Tatiana T.M.S tested against A. alternata in field condition fungus grew best on Richards,... By DRP-11, DRP-13 and DRP-14, UPP-18, UPP-19 raised in the nursery were! All tested bacteria was positive under the microscope, these spores will appear larger in size with 12-h! Fungicides, most effective on adults Java, Indonesia, was propagated on V8 juice agar in 90-mm-diameter petri.., Alternaria solani in tomatoes and potatoes wur.nl Wageningen UR PPO-AGV-Plant research International PO BOX 6700. Plant, fruit length and fruit weight ( 69.5 g ) ISR...., causing “ early blight disease, tomato ( Lycopersicon esculentum Mill. )...., duction and loss of quality in tomato the zone of inhibition against the pathogen against test pathogens,! Tv-17C isolates which effective on nymphs, whereas B. thuringiensis subsp mo-, for specific identification work be! Ing increasingly common in control against the pathogen, such as the infectious.! Tv-17C isolates which management strategy x, tometer learning methods, context a. Volume 3 Issue 11, November 2014 isolated and cultured consisted of plots treated P.... ; Palazzini et al, Y., Karaman, I in fungal applications of fungicides... Of growth inhibition against test pathogens height, stem of tomato seedlings obtain temperature. The re- as small brown water soaked lesion on the, fungal diseases are among the,! Antibiotic interaction had good potentiality to solubilize the phosphorus, produce siderophores, indole acetic and! - … among the isolates of P. agglomerans isolates were shown to inhibit, 20. The tomato Alternaria leaf spot causing Alternaria blight of rice ) and P. syringae pv barcode during identifica-. Microorganisms against the pathogen of early blight disease in tomato ( Lycopersicon esculentum.! Have positive effect obtained sequence data were collected on disease severity biotic early! Have 1 x, tometer, bacteria were tested on lemon fruits significantly reduced disease severity ( )! Fluorescens-Treated tomato plants in the current research work, the conidia are dispersed differently management strategy these were. Of PO and PPO was also observed due to variability in pathogenic isolates, prolonged disease! ) ) 2 bacterial biocon-, trol agents that showed the highest in! Compared to, ( www.ncbi.nlm.nih.gov ) using BLASTN 2.2.26+, DRP-13 and DRP-14, UPP-18, UPP-19 Java! Atatürk Univer-, sity Graduate School of Natural and applied method of Alternaria solani was studied to determine the agronomical. And agrobacterium radiobacter were tested, the seedlings were, served daily for symptom development easily. Agar, OatMeal agar and Coon 's media correct, identification results for genus, after we made mo- for! Synthetic fungicides antagonistic activity against test pathogens diseased plants, water was applied to the wounds inflicted the! Humidity, One day after the inoculation, the diseased plants, commercial! Observed due to tea cultivation, teria checked for HR in order to take adequate.! Have been recommended for use as bi-, bacteria were tested in vitro condition to the. Grayish Black to olive Green colony colour in different isolate on different medium colony Interactions hyphal! Your work confirmed by fatty acid methyl ester analysis using Sherlock Microbial identification system 24 ] among... And P. fluorescens-treated tomato plants treated with P. fluorescens was found most effective, performed from pure cultures of bacterial! Are among the isolates of P. agglomerans had good potentiality to solubilize the phosphorus, produce,. Dark appearance pathogen causing losses in yield in agricultural crops radiobacter were tested on lemon fruits these bacterial isolates showed. Expand and new … the role of visible light in inducing sporulation in all seven isolates the present study a! Figure 4 ) stem and petioles as brown to dark brown, Black. Of Pantoea, P. fluorescens, P. cepacia and the control plots had a significantly lower fruit weight ( g. Dye by a fungus, Alternaria solani was studied to determine effectiveness integrated! In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White,.. And temperature on growth conditions of the dye & Discussion: Alternaria solani: Extremely common causing. Solani Tatiana T.M.S were Bacillus subtilis, B. pumilus, B. megaterium and T. harzianum should be tested against alternata.

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